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I have left up last semester's review sheet as some students find these useful.
It will be updated for Fall 2010 2-5 days before the exam.
Exam 3 Review Sheet
Fall 2000
EXAM DATE: Friday December 4th
Material on exam:
Only the portions of the following chapters that are
listed on the syllabus and that we spoke about in class will be on the exam. If
their are pages on the syllabus that we did not discuss, it will not be on the
exam.
| Chapter
14 |
| Chapter
18 |
| Chapter
15 |
Chapter 14:
The Genetic Code and Transcription
Chapter 18: Regulation of Gene Expression in Eukaryotes
 | review the characteristics of the
genetic code (p. 353) |
| review the in-class handout which summarizes studies
done to determine characteristics of genetic code |
| review the in-class handout which summarizes studies
done to decipher the genetic code (homopolymer, mixed copolymer, repeating copolymers,
triplet binding assay). |
| Be able to answer
questions related to these experiments as in teh problem set.
|
| know how to determine amino acid sequence for a given
mRNA sequence (or starting from a given DNA sequence) using the genetics
code. The code will be provided. |
| What is the wobble hypothesis? Which position of the codon is affected?
Which position of the anticodon is affected?
|
| Review info in Table
14-7 |
| Prokaryotic Transcription See Figure
14-9
 | E. coli. RNA polymerase (a,
b, b',
and s subunits) |
| Which subunit(s) contains the active site and is responsible for the catalytic
activity of the enzyme? |
| Which subunit is a regulatory subunit involved in recognition of the promoter? |
| Prokaryotic promoter region includes a Pribnow box (-10) and sequences at -35 |
| Prokaryotic transcripts are polycistronic |
| transcription and translation simultaneous |
|
| Eukaryotic Transcription
| Eukaryotic transcripts are monocistronic |
| 3 different RNA polymerases:
 | RNA Pol I -- rRNA |
| RNA Pol II -- mRNA |
| RNA Pol III -- tRNA, 5S RNA |
|
| Transcription and translation are NOT simultaneous; occur in different parts of
the cell |
| More complex regulation |
| Processing: 5' cap, poly A tail, splicing. Where does each occur?
When? Why? |
| RNA Pol II (2 large subunits, 10-15 smaller) |
| What is the function of CAAT box, TATA box, enhancer? |
| TFIID, TBP |
| see p. 458-459, which summarizes differences between euk and
prok transcription |
| review section on
chromosome remodeling (Histone acetylation and DNA methylation). Why/how is this related to
transcription? |
| Figure 18-6, 18-7, eukaryotic promoter structure |
| Figure 18-14,Assembly of transcription factors |
| Figure 18-15, Enhancer protein model |
|
Chapter 15: Translation
| Figure 15-1, Ribosomes consist of small and
large subunits, made up of both proteins and RNA |
| Figure 15-3, tRNA structure (where is
anticodon loop? where is 5' end? 3' end? How does structure form?
Where does aa bind?) |
| Charging of tRNAs. Figure
15-5
| aminoacyl tRNA synthetase (what does this enzyme do?
How many different aminoacyl tRNA synthetases are there?) |
|
| Translation: Figures
15-6,
15-7 and
15-8
| you should be familiar with the general aspects of translation. You do not
need to know the specifics of initiation factors and elongation factors, but should know
that there are protein factors involved. |
| which amino acid is the first to be inserted during prokaryotic transcription? |
| where does the first amino acid sit in the ribosome? where does the second
amino acid sit in the ribosome? Where does the exiting amino acid sit in the
ribosome? |
| What is the Shine Delgarno sequence and what is its significance? |
| What is the function of peptidyl transferase? |
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