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Class Schedule:**

DATE

 

LECTURE TOPIC

Text

 

LAB TOPIC

Jan 23

Jan 25

 

WF

Course Introduction/ History of Biotechnology

Structure and properties of DNA: replication, denaturation, supercoiling, plasmids.

Ch1,2

Ch 3

23-35, H

1/24

1. Lab Safety,

Introduction to Laboratory Equipment and Procedures

Jan 28

Jan 30

Feb 1

M

W

F

 

Control of Gene Expression

Promoters/enhancers/activators/repressors

 

 

Ch 3.

38-45

1/31

2. Laboratory Calculations and Solution Making

Feb 4

Feb 6

 

Feb 8

M

W

 

F

LAL testing

Recombinant DNA Techniques: Restriction enzymes

 

Recombinant DNA Techniques: Cloning vectors

H

Ch 4

47-57

 

 

Ch 4

57-64;H

2/7

3. LAL testing

Feb 11

Feb 13

Feb 15

M

W

F

 

Transformation

LECTURE EXAM 1, Friday February 15

 

Ch 4

86-89

2/14

4. Restriction digest and analysis*

Feb 18

Feb 19

Feb 20

Feb 22

M

TW

F

No Classes; President’s Day

Construction and screening of libraries

Ch 4

64-86

2/21

5. Construction and cloning of a DNA recombinant.  Part 1*

·         ligation

·         transformation

Feb 25

 

Feb 27

Feb 29

M

 

W

F

Construction and screening of libraries, cont’d

 

Manipulation of prok.gene expression

Production of recomb. proteins in euk. cells

Ch 4

64-86

Ch.6

121-134

Ch.7, H

2/28

Construction and cloning of a DNA recombinant.  Part 2*

·         mini-preps

·         restriction digests

Mar 3

Mar 5

Mar 7

M

W

F

Production of recombinant proteins in eukaryotic cells

Recombinant DNA Techniques continued:

·         PCR

Ch 7, H

 

 

Ch 5

110-119

3/6

6. Spectrophotometer

PCR primers/Programming

DNA amplification by PCR

Mar 10

 

Mar 12

Mar 14

M

 

W

F

Recombinant DNA Techniques continued:

·         DNA Fingerprinting

·         Southern/Northern

LECTURE EXAM 2, Friday. March 14

 

H

3/13

7. PCR-based VNTR Human DNA Typing*

SPRING BREAK

Mar 24

Mar 26

Mar 28

M

W

F

Recombinant DNA Techniques continued:

·         Sequencing

·         ELISA

Ch 5.

101-110

H, Ch 9

227-242

3/27

8. Southern blot analysis (DNA Fingerprinting).Part 1

Mar 31

Apr 2

Apr 4

M

W

F

Large Scale Production of recombinant proteins

Transgenic Animals

Ch 16

481-506

 

Ch 19

4/3

9. Southern blot analysis (DNA Fingerprinting) *.  Part 2

Apr 7

Apr 9

Apr 11

M

W

F

Transgenic Animals

 

Bacterial Identification lab

 

 

Ch 17

4/10

Biolog system: Bacterial Identification/Fermentation

Apr 14

Apr 16

Apr 18

 

W

F

Plant Biotechnology

 

LECTURE EXAM 3, Friday April 18

Ch 17

H

4/17

10. ELISA*/Microarray

 

Apr 21

Apr 22

Apr 23

Apr 25

M

T

W

F

No Classes; President’s Day

Bioinformatics

 

Biotechnology Advisory Board Luncheon

 

H

4/24

11. Bioinformatics

Apr 28

Apr 30

May 2

M

W

F

GMOs

 

Selected Topics

Ch. 21

673-688

H

5/1

12. DNA unknowns

 

May 5

May 7

M

W

Selected topics

Selected Topics

H

 

NO LAB

 

May 12-15 Final Exam Week

**This schedule may change during the course of the semester; you will only be responsible for the material we discuss in class for exams.  The lab schedule is not expected to change.  H = handout from another source.  Labs indicated with * are labs that will have written lab reports.  Other labs may have worksheets or other short written assignments to be completed.